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biacore cm5 sensor chip (Biacore)

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Biacore biacore cm5 sensor chip
Biacore Cm5 Sensor Chip, supplied by Biacore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biacore+cm5+sensor+chip/chip+cm5+sensor/pm41944539-67-4-4
Average 86 stars, based on 1 article reviews

biacore cm5 sensor chip - by Bioz Stars, 2026-09

86/100 stars

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Recombinant:

Article Title: Direct Targeting of STAT3 by Xanthatin Suppresses Allergic Airway Inflammation via Inhibition of TSLP Release and NK2 Cell Polarization.
Article Snippet: .. Recombinant human STAT3 was fixed on a Biacore CM5 sensor chip, and the Jo urn al Pr e-p roo f remaining binding sites were blocked by 1 M ethanolamine (pH 8.5). ..

Article Title: Manipulating metabolism-reprogrammed monocytic-MDSCs prevents colitis-associated dysplasia by IL-10/HIF-1α/DLL4 signaling.
Article Snippet: SPR assay was performed by using the Biacore T200 (GE Healthcare, Chicago, IL, USA) as follows. .. Briefly, the recombinant human DLL4 protein was immobilized on a Biacore CM5 sensor chip via EDC/NHSmediated cross-linking reaction. ..

Binding Assay:

Concentration Assay:

Article Title: Identification of a small molecule Cyclophilin D inhibitor for rescuing Aβ-mediated mitochondrial dysfunction
Article Snippet: .. CypD protein was first dissolved in PBS (pH 5.0) at a concentration of 10 μg/ml, and then immobilized directly and covalently attached to a hydrophilic carboxy-methylated dextran matrix of the Biacore CM5 sensor chip using the standard primary amine coupling reaction and standard procedures. ..

SPR Assay:

Article Title: Kinetic Insights into Cholera Toxin B‑Biomimetic Glycan Interactions
Article Snippet: The control molecules for SPR, GM 1 pentasaccharide sodium salt, and Lewis Y trisaccharide were purchased from Enzo Life Sciences and Sigma-Aldrich, respectively. .. SPR materials, including a Biacore CM5 sensor chip, an amide coupling kit, 10 mM Na-acetate immobilization buffer, and P20 surfactant, were purchased from Cytiva. ..

Article Title: Kinetic Insights into Cholera Toxin B-Biomimetic Glycan Interactions.
Article Snippet: The control molecules for SPR, GM1 pentasaccharide sodium salt, and Lewis Y trisaccharide were purchased from Enzo Life Sciences and SigmaAldrich, respectively. .. SPR materials, including a Biacore CM5 sensor chip, an amide coupling kit, 10 mM Na-acetate immobilization buffer, and P20 surfactant, were purchased from Cytiva. ..

Solvent:

Article Title: Supporting Information Potent, selective and CNS-penetrant tetrasubstituted cyclopropane class IIa histone deacetylase (HDAC) inhibitors
Article Snippet: .. Solvent correction sequence every 48 cycles, including at the beginning and end of the run Preparation and conditioning of Biacore CM5 sensor chip With HBS-N as the run buffer, a new CM5 sensor chip was inserted into the Biacore T200 and the instrument was primed. ..

Sequencing:

other:

Article Title: Non-human animals expressing pH-sensitive immunoglobulin sequences
Article Snippet: Plates were then blocked with 250 μl of 0.5% bovine serum albumin (BSA, Sigma-Aldrich) in PBS and incubated for 1 hour at room temperature.

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Binding analysis between Nb1 and FtsB . A , sensorgrams of the analysis by SPR of the binding of Nb1 at 0.125, 0.25, 0.5, 1, or 2 nM against FtsB immobilized by amine coupling in the surface of a CM5 chip. Sensorgrams were measured by multicycle kinetics, in PBS-T (0.005%) with 300 s of association and 1200 s of dissociation, followed by regeneration with 1 M L-Gly pH 3.0 for 30 s. The thick colored lines represent the raw sensorgrams and the thin black lines are the fittings. The analyses were performed with the Biacore T200 Evaluation Software (Cytiva) using a 1:1 binding kinetic fitting model. B , Western blotting analysis of the binding of Nb1 to either recombinant FtsB ( left ) or recombinant FhuD2 ( right ). Transferred membranes were first treated with a 0.1 mg/ml solution of Nb1 in PBS-T (0.05%) for 1 h, followed by 45 min of incubation with a commercial goat anti-Alpaca VHH antibody conjugated with HRP (1:5000 dilution in PBS-T (0.05%)). For CBB and Western blotting, experiments with FtsB and FhuD2 were run in parallel in the same gel using a single marker lane. Therefore, the same molecular weight marker is shown for both proteins. SPR, surface plasmon resonance.

Journal: The Journal of Biological Chemistry

Article Title: Development of an inhibitory monoclonal nanobody targeting Streptococcus pyogenes siderophore binding protein FtsB

doi: 10.1016/j.jbc.2026.111224

Figure Lengend Snippet: Binding analysis between Nb1 and FtsB . A , sensorgrams of the analysis by SPR of the binding of Nb1 at 0.125, 0.25, 0.5, 1, or 2 nM against FtsB immobilized by amine coupling in the surface of a CM5 chip. Sensorgrams were measured by multicycle kinetics, in PBS-T (0.005%) with 300 s of association and 1200 s of dissociation, followed by regeneration with 1 M L-Gly pH 3.0 for 30 s. The thick colored lines represent the raw sensorgrams and the thin black lines are the fittings. The analyses were performed with the Biacore T200 Evaluation Software (Cytiva) using a 1:1 binding kinetic fitting model. B , Western blotting analysis of the binding of Nb1 to either recombinant FtsB ( left ) or recombinant FhuD2 ( right ). Transferred membranes were first treated with a 0.1 mg/ml solution of Nb1 in PBS-T (0.05%) for 1 h, followed by 45 min of incubation with a commercial goat anti-Alpaca VHH antibody conjugated with HRP (1:5000 dilution in PBS-T (0.05%)). For CBB and Western blotting, experiments with FtsB and FhuD2 were run in parallel in the same gel using a single marker lane. Therefore, the same molecular weight marker is shown for both proteins. SPR, surface plasmon resonance.

Article Snippet: FtsB (WT or mutants) and FhuD2 were immobilized in the channels of a CM5 Biacore sensor chip at 250 response units in 10 mM acetate pH 5.5 using the amine-coupling reaction, according to the manufacturer’s instructions.

Techniques: Binding Assay, Software, Western Blot, Recombinant, Incubation, Marker, Molecular Weight, SPR Assay

Epitope mapping of the Nb1–FtsB interaction . A and B , residual plots of the deuterium uptake scores of Fe(III)-FCH bound to FtsB ( A ) or Nb1-bound FtsB ( B ) relative to the unbound protein, obtained in HDX-MS experiments. The peptide coverage, and the domains of FtsB that each peptide and amino acid belong to, are indicated above the plot. Four regions of interest, showing changes in deuterium uptake between the unbound protein and the FCH-bound or Nb1-bound protein (regions 1–4) appear highlighted in red , blue , purple , and orange , respectively. C , deuterium uptake kinetics of individual peptides representative of region 1 ( left ), region 2 ( middle left ), region 3 ( middle right ), or region 4 ( right ). The curves represent unbound FtsB ( blue ), Nb1-bound FtsB ( red ), and FCH-bound FtsB ( green ). The sequence of each peptide is included above the graph, highlighting the amino acids known to participate in siderophore binding. D , sensorgrams of the analysis by SPR of the binding of Nb1 at 0.125, 0.25, 0.5, 1, or 2 nM against FtsB Y81A ( left ), Y137A ( middle left ), W204A ( middle right ), and Y232A ( right ) immobilized by amine coupling in the surface of a CM5 chip. Kinetics were measured with 300 s of association and 1200 s of dissociation. The thick colored lines represent the raw sensorgrams and the thin black lines the fittings, performed with the Biacore T200 Evaluation Software (Cytiva) using a 1:1 binding kinetic fitting model. FCH, ferrichrome; HDX-MS, hydrogen/deuterium exchange coupled with mass spectrometry; SPR, surface plasmon resonance.

Journal: The Journal of Biological Chemistry

Article Title: Development of an inhibitory monoclonal nanobody targeting Streptococcus pyogenes siderophore binding protein FtsB

doi: 10.1016/j.jbc.2026.111224

Figure Lengend Snippet: Epitope mapping of the Nb1–FtsB interaction . A and B , residual plots of the deuterium uptake scores of Fe(III)-FCH bound to FtsB ( A ) or Nb1-bound FtsB ( B ) relative to the unbound protein, obtained in HDX-MS experiments. The peptide coverage, and the domains of FtsB that each peptide and amino acid belong to, are indicated above the plot. Four regions of interest, showing changes in deuterium uptake between the unbound protein and the FCH-bound or Nb1-bound protein (regions 1–4) appear highlighted in red , blue , purple , and orange , respectively. C , deuterium uptake kinetics of individual peptides representative of region 1 ( left ), region 2 ( middle left ), region 3 ( middle right ), or region 4 ( right ). The curves represent unbound FtsB ( blue ), Nb1-bound FtsB ( red ), and FCH-bound FtsB ( green ). The sequence of each peptide is included above the graph, highlighting the amino acids known to participate in siderophore binding. D , sensorgrams of the analysis by SPR of the binding of Nb1 at 0.125, 0.25, 0.5, 1, or 2 nM against FtsB Y81A ( left ), Y137A ( middle left ), W204A ( middle right ), and Y232A ( right ) immobilized by amine coupling in the surface of a CM5 chip. Kinetics were measured with 300 s of association and 1200 s of dissociation. The thick colored lines represent the raw sensorgrams and the thin black lines the fittings, performed with the Biacore T200 Evaluation Software (Cytiva) using a 1:1 binding kinetic fitting model. FCH, ferrichrome; HDX-MS, hydrogen/deuterium exchange coupled with mass spectrometry; SPR, surface plasmon resonance.

Techniques: Sequencing, Binding Assay, Software, Mass Spectrometry, SPR Assay

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biacore cm5 sensor chip (Biacore)

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